2024Tools for editing the mammalian mitochondrial genome.
Moraes CT.Hum Mol Genet. 2024 May 22;33(R1):R92-R99. Absence of both MGME1 and POLG EXO abolishes mtDNA whereas absence of either creates unique mtDNA duplications.
Gonzalez CD, Nissanka N, Van Booven D, Griswold AJ, Moraes CT.J Biol Chem. 2024 Apr;300(4):107128. doi: 10.1016/j.jbc.2024.107128. mitoTALEN reduces the mutant mtDNA load in neurons.
Bacman SR, Barrera-Paez JD, Pinto M, Van Booven D, Stewart JB, Griswold AJ, Moraes CT.Mol Ther Nucleic Acids. 2024 Feb 2;35(1):102132. 2023Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease.
Shoop WK, Lape J, Trum M, Powell A, Sevigny E, Mischler A, Bacman SR, Fontanesi F, Smith J, Jantz D, Gorsuch CL, Moraes CT.Nat Metab. 2023 Dec;5(12):2169-2183. Mitochondrial dysfunction characterized in human induced pluripotent stem cell disease models in MELAS syndrome: A brief summary.
Latchman K, Saporta M, Moraes CT.Mitochondrion. 2023 Sep;72:102-105 A Defect in Mitochondrial Complex III but Not in Complexes I or IV Causes Early β-Cell Dysfunction and Hyperglycemia in Mice.
Lang AL, Nissanka N, Louzada RA, Tamayo A, Pereira E, Moraes CT, Caicedo A.Diabetes. 2023 Sep 1;72(9):1262-1276 2022Precise and simultaneous quantification of mitochondrial DNA heteroplasmy and copy number by digital PCR.
Shoop WK, Gorsuch CL, Bacman SR, Moraes CT.J Biol Chem. 2022 Nov;298(11):102574 Adult-Onset Deficiency of Mitochondrial Complex III in a Mouse Model of Alzheimer's Disease Decreases Amyloid Beta Plaque Formation.
Pinto M, Diaz F, Nissanka N, Guastucci CS, Illiano P, Brambilla R, Moraes CT.Mol Neurobiol. 2022 Oct;59(10):6552-6566. Mitochondrial genome engineering coming-of-age.
Barrera-Paez JD, Moraes CT.Trends Genet. 2022 Aug;38(8):869-880. 2021ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly.
Arguello T, Peralta S, Antonicka H, Gaidosh G, Diaz F, Tu YT, Garcia S, Shiekhattar R, Barrientos A, Moraes CT.Cell Rep. 2021 Dec 21;37(12):110139 Enhanced glycolysis and GSK3 inactivation promote brain metabolic adaptations following neuronal mitochondrial stress.
Garcia S, Saldana-Caboverde A, Anwar M, Raval AP, Nissanka N, Pinto M, Moraes CT, Diaz F.Hum Mol Genet. 2021 2020Myopathy reversion in mice after restauration of mitochondrial complex I.
Pereira CV, Peralta S, Arguello T, Bacman SR, Diaz F, Moraes CT. EMBO Mol Med. 2020 Jan 9:e10674. Metformin delays neurological symptom onset in a mouse model of neuronal complex I deficiency. Peralta S, Pinto M, Arguello T, Garcia S, Diaz F, Moraes CT. JCI Insight. 2020 Nov 5;5(21):141183 Mitochondrial DNA Base Editing: Good Editing Things Still Come in Small Packages. Bacman SR, Moraes CT. Mol Cell. 2020 Sep 3;79(5):708-709. 2019Genetics: Segregation of Mitochondrial Genomes in the Germline.
Moraes CT. Curr Biol. 2019 Aug 5;29(15):R746-R748. Sorting mtDNA Species-the Role of nDNA-mtDNA Co-evolution. Moraes CT. Cell Metab. 2019 Dec 3;30(6):1002-1004. Mechanisms of Mitochondrial DNA Deletion Formation. Nissanka N, Minczuk M, Moraes CT. Trends Genet. 2019 Mar;35(3):235-244. Photobiomodulation enhancement of cell proliferation at 660 nm does not require cytochrome c oxidase. Lima PLV, Pereira CV, Nissanka N, Arguello T, Gavini G, Maranduba CMDC, Diaz F, Moraes CT. J Photochem Photobiol B. 2019 May;194:71-75. doi: 10.1016/j.jphotobiol.2019.03.015. Ablation of Cytochrome c in Adult Forebrain Neurons Impairs Oxidative Phosphorylation Without Detectable Apoptosis. Pinto M, Vempati UD, Diaz F, Peralta S, Moraes CT. Mol Neurobiol. 2019 May:56:3722-3735. Representative Collaborative PublicationsTreatment with ROS detoxifying gold quantum clusters alleviates the functional decline in a mouse model of Friedreich ataxia.
Villa C, Legato M, Umbach A, Riganti C, Jones R, Martini B, Boido M, Medana C, Facchinetti I, Barni D, Pinto M, Arguello T, Belicchi M, Fagiolari G, Liaci C, Moggio M, Ruffo R, Moraes CT, Monguzzi A, Merlo GR, Torrente Y.Sci Transl Med. 2021 Aug 18;13(607) Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.
Protasoni M, Pérez-Pérez R, Lobo-Jarne T, Harbour ME, Ding S, Peñas A, Diaz F, Moraes CT, Fearnley IM, Zeviani M, Ugalde C, Fernández-Vizarra E. EMBO J. 2020 Jan 8:e102817. doi: 10.15252/embj.2019102817. Mitochondrial DNA Double-Strand Breaks in Oligodendrocytes Cause Demyelination, Axonal Injury, and CNS Inflammation. Madsen PM, Pinto M, Patel S, McCarthy S, Gao H, Taherian M, Karmally S, Pereira CV, Dvoriantchikova G, Ivanov D, Tanaka KF, Moraes CT, Brambilla R. J Neurosci. 2017 Oct 18;37(42):10185-10199. The CoQH2/CoQ Ratio Serves as a Sensor of Respiratory Chain Efficiency. Guarás A, Perales-Clemente E, Calvo E, Acín-Pérez R, Loureiro-Lopez M, Pujol C, Martínez-Carrascoso I, Nuñez E, García-Marqués F, Rodríguez-Hernández MA, Cortés A, Diaz F, Pérez-Martos A, Moraes CT, Fernández-Silva P, Trifunovic A, Navas P, Vazquez J, Enríquez JA. Cell Rep. 2016 Apr 5;15(1):197-209. Enhanced Transcriptional Activity and Mitochondrial Localization of STAT3 Co-induce Axon Regrowth in the Adult Central Nervous System. Luo X, Ribeiro M, Bray ER, Lee DH, Yungher BJ, Mehta ST, Thakor KA, Diaz F, Lee JK, Moraes CT, Bixby JL, Lemmon VP, Park KK. Cell Rep. 2016 Apr 12;15(2):398-410. Selective elimination of mitochondrial mutations in the germline by genome editing. Reddy P, Ocampo A, Suzuki K, Luo J, Bacman SR, Williams SL, Sugawara A, Okamura D, Tsunekawa Y, Wu J, Lam D, Xiong X, Montserrat N, Esteban CR, Liu GH, Sancho-Martinez I, Manau D, Civico S, Cardellach F, Del Mar O'Callaghan M, Campistol J, Zhao H, Campistol JM, Moraes CT, Izpisua Belmonte JC. Cell. 2015 Apr 23;161(3):459-69. |
2018The mitochondrial DNA polymerase gamma degrades linear DNA fragments precluding the formation of deletions.
Nissanka N, Bacman SR, Plastini MJ, Moraes CT. Nat Commun. 2018 Jun 27;9(1):2491. Lack of Parkin anticipates the phenotype and affects mitochondrial morphology and mtDNA levels in a mouse model of Parkinson's Disease.
Pinto M, Nissanka N, Moraes CT. J Neurosci. 2018 38(4):1042-1053 Overexpression of PGC-1α in aging muscle enhances a subset of young-like molecular patterns.
Garcia S, Nissanka N, Mareco EA, Rossi S, Peralta S, Diaz F, Rotundo RL, Carvalho RF, Moraes CT. Aging Cell. 2018 Apr;17(2). doi: 10.1111/acel.12707. ATAD3 controls mitochondrial cristae structure in mouse muscle, influencing mtDNA replication and cholesterol levels.
Peralta S, Goffart S, Williams SL, Diaz F, Garcia S, Nissanka N, Area-Gomez E, Pohjoismäki J, Moraes CT. J Cell Sci. 2018 Jul 4;131(13). pii: jcs217075. MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation.
Bacman SR, Kauppila JHK, Pereira CV, Nissanka N, Miranda M, Pinto M, Williams SL, Larsson NG, Stewart JB, Moraes CT. Nat Med. 2018 Nov;24(11):1696-1700. Mitochondrial methionyl N-formylation affects oxidative phosphorylation complexes steady-state levels and their organization into supercomplexes. Arguello T, Köhrer C, RajBhandary UL, Moraes CT. J Biol Chem. 2018 Aug 7. pii: jbc.RA118.003838. doi: 10.1074/jbc.RA118.003838. mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels. Pereira CV, Bacman SR, Arguello T, Zekonyte U, Williams SL, Edgell DR, Moraes CT. EMBO Mol Med. 2018 Jul 16. pii: e8084. doi: 10.15252/ emmm.201708084. 2017Transient mitochondrial DNA double strand breaks in mice cause accelerated aging phenotypes in a ROS-dependent but p53/p21-independent manner.
Pinto M, Pickrell AM, Wang X, Bacman SR, Yu A, Hida A, Dillon LM, Morton PD, Malek TR, Williams SL, Moraes CT. Cell Death Differ. 2017 Feb;24(2):288-299. Current strategies towards therapeutic manipulation of mtDNA heteroplasmy.
Pereira CV, Moraes CT. Front Biosci (Landmark Ed). 2017 Jan 1;22:991-1010. Review. 2016Sustained AMPK activation improves muscle function in a mitochondrial myopathy mouse model by promoting muscle fiber regeneration.
Peralta S, Garcia S, Yin HY, Arguello T, Diaz F, Moraes CT. Hum Mol Genet. 2016 Aug 1;25(15):3178-3191. Pioglitazone ameliorates the phenotype of a novel Parkinson's disease mouse model by reducing neuroinflammation.
Pinto M, Nissanka N, Peralta S, Brambilla R, Diaz F, Moraes CT. Mol Neurodegener. 2016 Apr 2;11(1):25. 2015Cre recombinase activity is inhibited in vivo but not ex vivo by a mutation in the asymmetric spacer region of the distal loxP site.
Arguello T, Moraes CT. Genesis. 2015 Nov;53(11):695-700. MitoTALEN: A General Approach to Reduce Mutant mtDNA Loads and Restore Oxidative Phosphorylation Function in Mitochondrial Diseases. Hashimoto M, Bacman SR, Peralta S, Falk MJ, Chomyn A, Chan DC, Williams SL, Moraes CT. Mol Ther. 2015 Oct;23(10):1592-9. Mechanisms linking mtDNA damage and aging. Pinto M, Moraes CT. Free Radic Biol Med. 2015 Aug;85:250-8. 2014The use of mitochondria-targeted endonucleases to manipulate mtDNA.
Bacman SR, Williams SL, Pinto M, Moraes CT. Methods Enzymol. 2014;547:373-97. Manipulating mitochondrial genomes in the clinic: playing by different rules. Moraes CT, Bacman SR, Williams SL. Trends Cell Biol. 2014 Apr;24(4):209-11. A magic bullet to specifically eliminate mutated mitochondrial genomes from patients' cells. Moraes CT. EMBO Mol Med. 2014 Apr;6(4):434-5. Mitochondrial genome changes and neurodegenerative diseases. Pinto M, Moraes CT. Biochim Biophys Acta. 2014 Aug;1842(8):1198-207 Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage. Peralta S, Torraco A, Wenz T, Garcia S, Diaz F, Moraes CT. Hum Mol Genet. 2014 Mar 15;23(6):1399-412. 2013Somatic mtDNA mutation spectra in the aging human putamen.
Williams SL, Mash DC, Züchner S, Moraes CT. PLoS Genet. 2013;9(12):e1003990. doi: Translational research in primary mitochondrial diseases: challenges and opportunities. Moraes CT, Anderson V, Mohan C; Workshop participants. Mitochondrion. 2013 Nov;13(6):945-52. Specific elimination of mutant mitochondrial genomes in patient-derived cells by mitoTALENs. Bacman SR, Williams SL, Pinto M, Peralta S, Moraes CT. Nat Med. 2013 Sep;19(9):1111-3. Mitochondrial alterations during carcinogenesis: a review of metabolic transformation and targets for anticancer treatments. Wang X, Peralta S, Moraes CT. Adv Cancer Res. 2013;119:127-60. Transient systemic mtDNA damage leads to muscle wasting by reducing the satellite cell pool. Wang X, Pickrell AM, Rossi SG, Pinto M, Dillon LM, Hida A, Rotundo RL, Moraes CT. Hum Mol Genet. 2013 Oct 1;22(19):3976-86. Mitochondrial DNA damage in a mouse model of Alzheimer's disease decreases amyloid beta plaque formation. Pinto M, Pickrell AM, Fukui H, Moraes CT. Neurobiol Aging. 2013 Oct;34(10):2399-407. |
